| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation
|
| Autores: |
Slivinski,Christiane Trevisan
Machado,Alex Vinicius Lopes
Iulek,Jorge
Ayub,Ricardo Antônio
Almeida,Mareci Mendes de
|
| Data: |
2011-06-01
|
| Ano: |
2011
|
| Palavras-chave: |
Glucoamylase
Aspergillus niger
Solid-state fermentation
Biochemical characterization
Purification
|
| Resumo: |
In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was inadvisable. K M and Vmax were 0.34 mg mL-1 and 160.22 U mL-1, respectively.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132011000300018
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.54 n.3 2011
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
