| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
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| Autores: |
Santos,Neyliane Gonçalves dos
Amaral,Ademir
Cavalcanti,Mariana Brayner
Neves,Maria Amélia Batista
Machado,Cíntia Gonsalves de Faria
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| Data: |
2008-12-01
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| Ano: |
2008
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| Palavras-chave: |
Combined therapy
Radiation effect
P-glycoprotein
Multidrug resistance
Functional analysis
Flow cytometry
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| Resumo: |
The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in combined chemo-and radiotherapy, particularly when radiation exposure precedes chemical treatment.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132008000700018
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.51 n.spe 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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