| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Determination of eye irritation potential of low-irritant products: comparison of in vitro results with the in vivo draize rabbit test
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| Autores: |
Nóbrega,Andréa Martins da
Alves,Eloisa Nunes
Presgrave,Rosaura de Farias
Costa,Rodrigo Netto
Delgado,Isabella Fernandes
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| Data: |
2012-06-01
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| Ano: |
2012
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| Palavras-chave: |
Alternative methods
Eye irritation
Draize Test
Low-irritant
HET-CAM
RBC
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| Resumo: |
In an attempt to build the evaluation strategies to assess the human eye irritation, a reassessment of some in vitro tests is necessary, particularly concerning the non-irritants, mild and moderate irritants. Thus, the correlations between results obtained from the Draize test with the in vitro methods HET-CAM (Hens Egg Test-Chorion Allantonic Membrane) and RBC haemolysis assay to assess the ocular irritancy potential of 20 eye drops were examined. Parameters such as accuracy (%) and specificity (%) were determined. All results were correlated with RBC correctly with the results obtained in the Draize test. The HET-CAM presented four false-positive results, showing a tendency of data overestimation. Despite the high specificity provided mainly by the RBC, it would be necessary to test a wider range of products representing all the scales of irritation to confirm its ability to be used as a first alternative to test products that could be presumptive non-irritants.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300008
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132012000300008
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.55 n.3 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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