| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Hydrolysis of whey lactose by immobilized β-Galactosidase
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| Autores: |
Mariotti,Marcela Panaro
Yamanaka,Hideko
Araujo,Angela Regina
Trevisan,Henrique Celso
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| Data: |
2008-12-01
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| Ano: |
2008
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| Palavras-chave: |
Β-galactosidase
Immobilization
Lactase
Whey
Lactose
Hydrolysis
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| Resumo: |
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132008000600019
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.51 n.6 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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