| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Rice bran as a substrate for proteolytic enzyme production
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| Autores: |
Sumantha,Alagarsamy
Deepa,Paul
Sandhya,Chandran
Szakacs,George
Soccol,Carlos Ricardo
Pandey,Ashok
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| Data: |
2006-09-01
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| Ano: |
2006
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| Palavras-chave: |
Solid-state fermentation
Rice bran
Rhizopus
Neutral protease
Agro-industrial residues
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| Resumo: |
Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme purity was achieved in this manner. The enzyme was found to be a metalloprotease, being activated by Mn2+, with maximal activity at a temperature of 60 C and pH 7.0.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600019
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132006000600019
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.49 n.5 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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