| Registro completo |
| Provedor de dados: |
Bragantia
|
| País: |
Brazil
|
| Título: |
Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity
|
| Autores: |
Oliveira,Érica Cristina de
Pinto-Maglio,Cecília Alzira Ferreira
|
| Data: |
2017-12-01
|
| Ano: |
2017
|
| Palavras-chave: |
Triticum aestivum
Triticum turgidum ssp. durum
Karyotype
CMA/DAPI banding
FISH-rDNA
Cytomolecular mapping
|
| Resumo: |
ABSTRACT Karyotyping and chromosome complement knowledge are a prerequisite for cytogenetic mapping. The aim of this study was to characterize 1 common wheat cultivar (BH 1146) and 4 durum wheat genotypes (IAC 1003, Langdon, P19 and P33) from the breeding program of the Agronomic Institute of Campinas (IAC), using karyotype analysis together with Chromomycin A3/4’-6-Diamidino-2-phenylindole (CMA/DAPI) banding techniques for longitudinal characterization of chromosomes and fluorescence in situ hybridization (FISH) in the mapping of the 45S rDNA region. Durum wheat chromosome complement number was 2n = 4x = 28 with the following karyotype formula: 22m + 6sm. Common wheat chromosome complement number was 2n = 6x = 42 with the following karyotype formula: 34m + 8sm. Tetraploid and hexaploid genomes presented 2 pairs of chromosomes with secondary constriction and terminal satellites. All 5 wheat genotypes exhibited CMA and DAPI bands and signals in the 45S rDNA regions (FISH-probe pTa71) which varied in number and location on the chromosome complement. All lines showed greater amount of DAPI bands than CMA bands. The FISH-rDNA 45S in situ hybridization signals, related to the nucleolar organizing regions (NORs), were observed in common wheat, presenting signals for the 45S rDNA sequence in 3 pairs of chromosomes. Durum wheat presented signals in 2 pairs. The CMA bands coincided with the location of 45S rDNA region in the chromosomes of durum wheat. The techniques were efficient for chromosomal characterization of all 5 genotypes.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000400456
|
| Editor: |
Instituto Agronômico de Campinas
|
| Relação: |
10.1590/1678-4499.2016.278
|
| Formato: |
text/html
|
| Fonte: |
Bragantia v.76 n.4 2017
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
