| Registro completo |
| Provedor de dados: |
Braz. J. Genet.
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| País: |
Brazil
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| Título: |
TP53 mutations and loss of heterozygosity of chromosome 17 in colorectal tumors
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| Autores: |
Yamamoto,Lidia
Lopes,Ademar A.
Harb-Gama,Angelito
Nagai,Maria A.
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| Data: |
1996-01-01
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| Ano: |
1996
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| Palavras-chave: |
TP53 mutations
Heterozygosity
Chromosome 17
Colorectal tumors
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| Resumo: |
The incidence of TP53 point mutations and loss of heterozygosity (LOH) of chromosome 17 in colorectal tumors was determined in a group of Brazilian patients. We screened DNA samples from tumors and distal normal mucosa of 39 patients with colorectal cancer, for TP53 mutations by PCR-SSCP (single-strand conformation polymorphism) analysis. Chromosome 17 LOH was investigated using six PCR-based polymorphic markers and one VNTR probe. TP53 mutations were demonstrated in 15/39 of the cases. Mutations were distributed among all exons examined (five to eight), the majority of them being G/C to A/T transitions. LOH of chromosome 17p and 17q was detected in 70 and 46% of the tumors, respectively. There was a significant association between TP53 mutations and LOH in chromosome 17p (P = 0.0035) and 17q (P = 0.03). Although no correlation was observed between TP53 genetic alterations and clinical/ pathological characteristics, the association of TP53 mutations with loss of both chromosome 17 arms may indicate that TP53 inactivation provokes an unstable phenotype in tumor cells in colorectal tumors.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400022
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S0100-84551996000400022
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Genetics v.19 n.4 1996
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| Direitos: |
info:eu-repo/semantics/openAccess
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