| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing
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| Autores: |
d'Azevedo,Pedro A.
Gonçalves,Ana Lúcia S.
Musskopf,Maiara I.
Ramos,Carina G.
Dias,Cícero A. G.
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| Data: |
2004-10-01
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| Ano: |
2004
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| Palavras-chave: |
ESBL
Double disk confirmatory test
Cefoxitin susceptibility testing
Screening test
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| Resumo: |
Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of clavulanic acid inhibition effect (CAIE). Only 72/107 (67%) of the isolates were confirmed as ESBL producers by DDT, with diverse results among species. By the E-test, 58/107 (54%) isolates were confirmed as ESBL producers, and 18/107 (17%) were not determinable. Susceptibility to cefoxitin was found in 57/68 (83%) of strains that did not show CAIE. ESBL detection remains a controversial issue and clinical laboratories are in need of a simple and effective way to recognize strains with this kind of resistance.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500006
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702004000500006
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.8 n.5 2004
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| Direitos: |
info:eu-repo/semantics/openAccess
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