| Registro completo |
| Provedor de dados: |
BJID
|
| País: |
Brazil
|
| Título: |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
|
| Autores: |
Macedo,Carla Isabel
Carnieli Jr,Pedro
Brandão,Paulo Eduardo
Rosa,Elizabeth S. Travassos da
Oliveira,Rafael de Novaes
Castilho,Juliana Galera
Medeiros,Rita
Machado,Rosangela Rocha
Oliveira,Rosely Cerqueira de
Carrieri,Maria Luiza
Kotait,Ivanete
|
| Data: |
2006-10-01
|
| Ano: |
2006
|
| Palavras-chave: |
Human rabies
Neck-skin
Diagnosis
RT-PCR
DNA sequencing
|
| Resumo: |
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008
|
| Editor: |
Brazilian Society of Infectious Diseases
|
| Relação: |
10.1590/S1413-86702006000500008
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Infectious Diseases v.10 n.5 2006
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
