| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
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| Autores: |
Wang,Xia
Jiao,Junhua
Xu,Weihua
Chai,Xiaoyan
Li,Zhenyun
Wang,Qingjiang
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| Data: |
2013-12-01
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| Ano: |
2013
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| Palavras-chave: |
Mycobacterium tuberculosis
Multiplex allele specific polymerase chain reaction (MAS-PCR)
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| Resumo: |
OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600008
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1016/j.bjid.2013.04.008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.17 n.6 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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