| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica
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| Autores: |
Santos,Helena Lúcia Carneiro
Peralta,Regina Helena Saramago
Macedo,Heloisa Werneck de
Barreto,Magali Gonçalves Muniz
Peralta,José Mauro
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| Data: |
2007-06-01
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| Ano: |
2007
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| Palavras-chave: |
Multiplex-PCR
Entamoeba histolytica
Amebiasis
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| Resumo: |
Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated that only 27 (21%) samples were positive for E. histolytica/E. dispar complex. Among these stool samples, 11 were positive by Multiplex-PCR, with nine presenting the diagnostic fragment characteristic of E. dispar (96 bp) and two presenting diagnostic fragment of E. histolytica (132 bp). Among negative samples detected by microscopic examination, three positive samples for E. dispar and one positive for E. histolytica by Multiplex-PCR was observed. This denotes a low sensibility of microscopic examination when a single stool sample is analyzed. Assay for detection of E. histolytica antigen was concordant with multiplex-PCR in relation to E. histolytica. Statistical analysis comparing the sensibility tests was not done because of the low number of E. histolytica cases. The results demonstrate the importance of the specific techniques use for the differentiation between E. histolytica and E. dispar.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300013
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702007000300013
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.11 n.3 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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