| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Evaluation of the accuracy of various phenotypic tests to detect oxacillin resistance in coagulase-negative staphylococci
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| Autores: |
Perez,Leandro Reus Rodrigues
d'Azevedo,Pedro Alves
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| Data: |
2008-06-01
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| Ano: |
2008
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| Palavras-chave: |
Oxacillin
Cefoxitin
CoNS
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| Resumo: |
In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30µg-cefoxitin and 1µg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. Of these, 138 (78.4%) harbored the mecA gene. Using 30µg-cefoxitin and 1µg-oxacillin disks we obtained 100 and 98.3% accuracy, respectively. In addition, when cefoxitin was used as marker in an agar dilution method, the higher accuracy (99.4%) was established with 8mg cefoxitin per mL breakpoint. Thus, despite of the agar dilution method using cefoxitin as a marker not being standard for this detection, our results suggested that it is an excellent alternative to detect the oxacillin resistance/mecA gene among CoNS isolates.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300009
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702008000300009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.12 n.3 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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