| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells
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| Autores: |
Zhang,Yafei
Zhang,Bicheng
Zhang,Anran
Li,Xiaohua
Liu,Jian
Zhao,Jie
Zhao,Yong
Gao,Jianfei
Fang,Dianchun
Rao,Zhiguo
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| Data: |
2013-01-01
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| Ano: |
2013
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| Palavras-chave: |
MiR-26a
IL-6
C-Myc
Hepatocellular carcinoma
Carcinoma prognosis
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| Resumo: |
A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be a predictor of survival up to 5 years for hepatocellular carcinoma patients (log-rank test, P < 0.05). We observed that the serum IL-6 concentration was inversely correlated with miR-26a expression in cancerous tissues (Pearson correlation test, r = -0.651, P < 0.01). Furthermore, by in vitro experiments with HepG2 cells, we showed that IL-6 stimulation can lead to miR-26a suppression via c-Myc activation, whereas in normal hepatocyte LO2 cells incubation with IL-6 had no significant effect on miR-26a expression. Taken together, these results indicate that miR-26a reduction in hepatocellular carcinoma might be due to IL-6 upregulation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X2012007500155
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.46 n.1 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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