| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
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| Autores: |
Wang,X.M.
Gao,S.J.
Guo,X.F.
Sun,W.J.
Yan,Z.Q.
Wang,W.X.
Xu,Y.Q.
Lu,D.
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| Data: |
2014-04-01
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| Ano: |
2014
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| Palavras-chave: |
CIAPIN1 gene
Multidrug resistance
RNA interference
MDR1 gene
Breast neoplasms
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| Resumo: |
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400273
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/1414-431X20133356
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.47 n.4 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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