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Provedor de dados:  BJMBR
País:  Brazil
Título:  Erythrocyte glucose-6-phosphate dehydrogenase activity assay and affinity for its substrate under "physiological" conditions
Autores:  Leite,A.A.
Barretto,O.C. de O.
Data:  1998-12-01
Ano:  1998
Palavras-chave:  Glucose-6-phosphate dehydrogenase
Red blood cells
G6PD activity and affinity
Resumo:  Glucose-6-phosphate dehydrogenase (G6PD) activity and the affinity for its substrate glucose-6-phosphate were investigated under conditions similar to the physiological environment in terms of ionic strength (I: 0.188), cation concentration, pH 7.34, and temperature (37oC). A 12.4, 10.4 and 21.4% decrease was observed in G6PD B, G6PD A+ and G6PD A- activities, respectively. A Km increase of 95.1, 94.4 and 95.4% was observed in G6PD B, G6PD A+ and G6PD A-, respectively, leading to a marked decrease in affinity. In conclusion, the observation of the reduced activity and affinity for its natural substrate reflects the actual pentose pathway rate. It also suggests a much lower NADPH generation, which is crucial mostly in G6PD-deficient individuals, whose NADPH availability is poor.
Tipo:  Info:eu-repo/semantics/other
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200004
Editor:  Associação Brasileira de Divulgação Científica
Relação:  10.1590/S0100-879X1998001200004
Formato:  text/html
Fonte:  Brazilian Journal of Medical and Biological Research v.31 n.12 1998
Direitos:  info:eu-repo/semantics/openAccess
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