| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
The role of T cell subsets and cytokines in the regulation of intracellular bacterial infection
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| Autores: |
Oliveira,S.C.
Harms,J.S.
Rech,E.L.
Rodarte,R.S.
Bocca,A.L.
Goes,A.M.
Splitter,G.A.
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| Data: |
1998-01-01
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| Ano: |
1998
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| Palavras-chave: |
T cell subsets
Brucella abortus
Knockout mice
Cytokines
Intracellular pathogens
Genetic immunization
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| Resumo: |
Cellular immune responses are a critical part of the host's defense against intracellular bacterial infections. Immunity to Brucella abortus crucially depends on antigen-specific T cell-mediated activation of macrophages, which are the major effectors of cell-mediated killing of this organism. T lymphocytes that proliferate in response to B. abortus were characterized for phenotype and cytokine activity. Human, murine, and bovine T lymphocytes exhibited a type 1 cytokine profile, suggesting an analogous immune response in these different hosts. In vivo protection afforded by a particular cell type is dependent on the antigen presented and the mechanism of antigen presentation. Studies using MHC class I and class II knockout mice infected with B. abortus have demonstrated that protective immunity to brucellosis is especially dependent on CD8+ T cells. To target MHC class I presentation we transfected ex vivo a murine macrophage cell line with B. abortus genes and adoptively transferred them to BALB/c mice. These transgenic macrophage clones induced partial protection in mice against experimental brucellosis. Knowing the cells required for protection, vaccines can be designed to activate the protective T cell subset. Lastly, as a new strategy for priming a specific class I-restricted T cell response in vivo, we used genetic immunization by particle bombardment-mediated gene transfer
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100010
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X1998000100010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.31 n.1 1998
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| Direitos: |
info:eu-repo/semantics/openAccess
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