| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
B cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunization
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| Autores: |
Fontoura,I. C.
Trombone,A.P.F.
Almeida,L. P.
Lorenzi,J. C. C.
Rossetti,R. A. M.
Malardo,T.
Padilha,E.
Schluchting,W.
Silva,R. L. L.
Gembre,A. F.
Fiuza,J. E. C.
Silva,C. L.
Panunto-Castelo,A.
Coelho-Castelo,A. A. M.
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| Data: |
2015-12-01
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| Ano: |
2015
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| Palavras-chave: |
DNA-Hsp65 vaccine
Memory T cells
B cells
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| Resumo: |
In DNA vaccines, the gene of interest is cloned into a bacterial plasmid that is engineered to induce protein production for long periods in eukaryotic cells. Previous research has shown that the intramuscular immunization of BALB/c mice with a naked plasmid DNA fragment encoding the Mycobacterium leprae 65-kDa heat-shock protein (pcDNA3-Hsp65) induces protection against M. tuberculosis challenge. A key stage in the protective immune response after immunization is the generation of memory T cells. Previously, we have shown that B cells capture plasmid DNA-Hsp65 and thereby modulate the formation of CD8+ memory T cells after M. tuberculosis challenge in mice. Therefore, clarifying how B cells act as part of the protective immune response after DNA immunization is important for the development of more-effective vaccines. The aim of this study was to investigate the mechanisms by which B cells modulate memory T cells after DNA-Hsp65 immunization. C57BL/6 and BKO mice were injected three times, at 15-day intervals, with 100 µg naked pcDNA-Hsp65 per mouse. Thirty days after immunization, the percentages of effector memory T (TEM) cells (CD4+ and CD8+/CD44high/CD62Llow) and memory CD8+ T cells (CD8+/CD44high/CD62Llow/CD127+) were measured with flow cytometry. Interferon γ, interleukin 12 (IL-12), and IL-10 mRNAs were also quantified in whole spleen cells and purified B cells (CD43−) with real-time qPCR. Our data suggest that a B-cell subpopulation expressing IL-10 downregulated proinflammatory cytokine expression in the spleen, increasing the survival of CD4+ TEM cells and CD8+ TEM/CD127+ cells.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201095
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/1414-431x20154409
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.48 n.12 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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