| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Comparison of three decontamination methods for Mycobacterium bovis isolation
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| Autores: |
Ambrosio,Simone Rodrigues
Oliveira,Eugenia Márcia de Deus
Rodriguez,Cesar Alejandro Rosales
Ferreira Neto,José Soares
Amaku,Marcos
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| Data: |
2008-06-01
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| Ano: |
2008
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| Palavras-chave: |
Mycobacterium bovis
Decontamination
Petroff
HPC
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| Resumo: |
Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822008000200008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.39 n.2 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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