| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
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| Autores: |
Saxena,Rajshree
Singh,Rajni
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| Data: |
2011-12-01
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| Ano: |
2011
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| Palavras-chave: |
Solid State Fermentation
Agro-industrial wastes
Amylase
Enzyme optimization
Stability
Bacillus sp
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| Resumo: |
Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822011000400014
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.42 n.4 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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