| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
A practical method for screening for beta-galactosidase secreting microbial colonies
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| Autores: |
Barreto,Eriana S.
Silva,Daison O.
Passos,Flavia M. Lopes
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| Data: |
2000-03-01
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| Ano: |
2000
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| Palavras-chave: |
BIOTROL®
Beta-galactosidase
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| Resumo: |
Microbial colonies were replicated on YNB® agar plates overlaid with soft agar containing the glucose-oxidase/peroxidase (BIOTROL®) system. The pink color developed around the colonies was the result of the reaction of the glucose generated by the extracellular hydrolysis of lactose by beta-galactosidase, indicating secretion of this enzyme. This method proved to be very convenient for testing hundreds of colonies grown on agar plates for beta-galactosidase secretion by microbial cells.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100009
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822000000100009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.31 n.1 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
|
