| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection
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| Autores: |
Coutinho,Tania Alen
Bernardi,Mari Lourdes
Cardoso,Marisa Ribeiro de Itapema
Borowski,Sandra Maria
Moreno,Andrea Micke
Barcellos,David Emilio Santos Neves de
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| Data: |
2009-09-01
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| Ano: |
2009
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| Palavras-chave: |
Bordetella bronchiseptica
Swine
Transport media
Selective media
PCR
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| Resumo: |
Three comparative assays were performed seeking to improve the sensitivity of the diagnosis of Bordetella bronchiseptica infection analyzing swine nasal swabs. An initial assay compared the recovery of B. bronchiseptica from swabs simultaneously inoculated with B. bronchiseptica and some interfering bacteria, immersed into three transport formulations (Amies with charcoal, trypticase soy broth and phosphate buffer according to Soerensen supplemented with 5% of bovine fetal serum) and submitted to different temperatures (10ºC and 27ºC) and periods of incubation (24, 72 and 120 hours). A subsequent assay compared three selective media (MacConkey agar, modified selective medium G20G and a ceftiofur medium) for their recovery capabilities from clinical specimens. One last assay compared the polymerase chain reaction to the three selective media. In the first assay, the recovery of B. bronchiseptica from transport systems was better at 27ºC and the three formulations had good performances at this temperature, but the collection of qualitative and quantitative analysis indicated the advantage of Amies medium for nasal swabs transportation. The second assay indicated that MacConkey agar and modified G20G had similar results and were superior to the ceftiofur medium. In the final assay, polymerase chain reaction presented superior capability of B. bronchiseptica detection to culture procedures.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300009
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822009000300009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.40 n.3 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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