| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract
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| Autores: |
Jain,Sumat Chand
Jain,P.C.
Kango,Naveen
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| Data: |
2012-03-01
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| Ano: |
2012
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| Palavras-chave: |
Inulinase
Dahlia
Fructose
Kluyveromyces marxianus
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| Resumo: |
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL-1) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL-1). The yeast showed good growth on a simple medium containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and 120 rpm. Lowest inulinase yield (4.8 nkat mL-1) was seen in the medium containing glucose as C-source. Although varied inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various protein sources tested, yeast extract was found to be the best source followed by beef extract (17.9 nkat mL-1) and peptone (13.8 nkat mL-1). The enzyme was optimally active at pH (4.0) and 50°C. TLC analysis of end product revealed that inulinase hydrolyzed inulin exclusively into fructose. Results suggest that the dahlia extract induced exoinulinase synthesis in Kluyveromyces marxianus and can be utilized as a potential substrate for inulinase production.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100007
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822012000100007
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.43 n.1 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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