| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
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| Autores: |
Sun,Xinyi
Hao,Long
Ma,Hua
Li,Tong
Zheng,Lan
Ma,Zhao
Zhai,Guoyin
Wang,Liqin
Gao,Shanglong
Liu,Xiaonan
Jia,Mengshi
Jia,Le
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| Data: |
2013-12-01
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| Ano: |
2013
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| Palavras-chave: |
Pleurotus eryngii SI-02
Exopolysaccharide
Extraction
Antioxidant activity
In vitro
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| Resumo: |
The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822013000400009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.44 n.4 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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