| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
PARTIAL CHARACTERIZATION OF PROTEASES FROM STREPTOMYCES CLAVULIGERUS USING AN INEXPENSIVE MEDIUM
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| Autores: |
Moreira,Keila Aparecida
Cavalcanti,Maria Taciana Holanda
Duarte,Helena Simões
Tambourgi,Elias Basile
Melo,Eduardo Henrique Magalhães de
Silva,Valdinete Lins
Porto,Ana Lúcia Figueiredo
Lima Filho,José Luiz de
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| Data: |
2001-10-01
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| Ano: |
2001
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| Palavras-chave: |
Streptomyces clavuligerus
Extracellular proteases
Thermal stability
PH
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| Resumo: |
The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for enzymes from the partially purified extract 3585 and 644 strains, respectively. The optimum temperature for the crude extract was 21ºC for both strains. However, for the partially preparation the optimum temperature was 50ºC and 40°C for S. clavuligerus NRRL 3585 and 644 strains respectively.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822001000300010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.32 n.3 2001
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| Direitos: |
info:eu-repo/semantics/openAccess
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