| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
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| Autores: |
Rosário,Tatiana Reis do
Dib,Cristina Corsi
Roxo,Eliana
Pinheiro,Sônia Regina
Vasconcellos,Silvio Arruda
Benites,Nilson Roberti
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| Data: |
2014-01-01
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| Ano: |
2014
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| Palavras-chave: |
Tuberculosis
Mycobacterium bovis
Thin layer
PCR
Cattle and buffalo organs
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| Resumo: |
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822014005000038
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.45 n.1 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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