Registro completo |
Provedor de dados: |
BJM
|
País: |
Brazil
|
Título: |
Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc.)
|
Autores: |
Hayat,Sikandar
Christias,Christos
|
Data: |
2010-03-01
|
Ano: |
2010
|
Palavras-chave: |
Protoplast
Fusion
Sclerotium rolfsii
Pathogenicity and osmotic stabilizer
|
Resumo: |
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato.
|
Tipo: |
Info:eu-repo/semantics/article
|
Idioma: |
Inglês
|
Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100035
|
Editor: |
Sociedade Brasileira de Microbiologia
|
Relação: |
10.1590/S1517-83822010000100035
|
Formato: |
text/html
|
Fonte: |
Brazilian Journal of Microbiology v.41 n.1 2010
|
Direitos: |
info:eu-repo/semantics/openAccess
|