| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil
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| Autores: |
Gonzaga,Rosa Maria S.
Rodart,Itatiana F.
Reis,Mitermayer Galvão
Ramalho Neto,Cícero Eduardo
Silva,Denise Wanderlei
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| Data: |
2008-12-01
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| Ano: |
2008
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| Palavras-chave: |
Hepatitis C virus
5'NCR
Core region
RT-nested-PCR
Genotypes
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| Resumo: |
We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400008
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822008000400008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.39 n.4 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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