| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
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| Autores: |
Li,Jiang
Hu,Qiushi
Li,Yuquan
Xu,Yuan
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| Data: |
2015-09-01
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| Ano: |
2015
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| Palavras-chave: |
Psychrophilic bacteria
Cold-adapted agarase
Purification
Characterization
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| Resumo: |
An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300683
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-838246320131289
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.46 n.3 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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