| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Involvement of G proteins and cAMP in the production of chitinolytic enzymes by Trichoderma harzianum
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| Autores: |
Firmino,Alexandre A.P.
Ulhoa,Cirano J.
Sousa,Marcelo V.
Ferreira Filho,Edivaldo X.
Ricart,Carlos A.O.
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| Data: |
2002-06-01
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| Ano: |
2002
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| Palavras-chave: |
Trichoderma
N-acetylglucosaminidase
Chitinase
CAMP
G protein
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| Resumo: |
The effect of G protein modulators and cyclic AMP (cAMP) on N-acetylglucosaminidase (NAGase) production was investigated during 84 h of growth of a Trichoderma harzianum strain in chitin-containing medium. Caffeine (5 mM), N6--2'-O-dibutyryladenosine 3'5'-cyclic monophosphate sodium salt (dBcAMP) (1 mM) and 3-isobutyl-1-methylxanthine (IBMX) (2 mM) decreased extracellular NAGase activity by 80%, 77% and 37%, respectively. AlCl3/KF (100 µM/10 mM and 200 µM/ 20 mM) decreased the activity by 85% and 95%, respectively. Cholera (10 µ/mL) and pertussis (20 µ/mL) toxins also affected NAGase activity, causing a decrease of approximately 75%. Upon all treatments, protein bands of approximately 73 kDa, 68 kDa and 45 kDa had their signals diminished whilst a 50 kDa band was enhanced only by treatment with cholera and pertussis toxins. N-terminal sequencing analysis identified the 73 kDa and 68 kDa proteins as being T. harzianum NAGase in two different truncated forms whereas the 45 kDa band comprised a T. harzianum endochitinase. The 50 kDa protein showed sequence similarity to Coriolus vesicolor cellobiohydrolase. The above results suggest that a signaling pathway comprising G-proteins, adenylate cyclase and cAMP may be involved in the synthesis of T. harzianum chitinases.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200015
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822002000200015
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.33 n.2 2002
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| Direitos: |
info:eu-repo/semantics/openAccess
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