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Provedor de dados:  BJM
País:  Brazil
Título:  Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil
Autores:  Franco,Ana C.
Rijsewijk,Frans A.M.
Flores,Eduardo F.
Weiblen,Rudi
Roehe,Paulo M.
Data:  2002-09-01
Ano:  2002
Palavras-chave:  Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis
BoHV-1
Deletion mutant
Differential vaccine
Resumo:  This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co-transfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300018
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822002000300018
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.33 n.3 2002
Direitos:  info:eu-repo/semantics/openAccess
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