| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
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| Autores: |
Barense,Renata I.
Chellegatti,Maria Angélica dos S.C.
Fonseca,Maria José V.
Said,Suraia
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| Data: |
2001-12-01
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| Ano: |
2001
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| Palavras-chave: |
Exopolygalacturonase
Pectinase
Polygalacturonase
Penicillium frequentans
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| Resumo: |
Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822001000400014
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.32 n.4 2001
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| Direitos: |
info:eu-repo/semantics/openAccess
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