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Provedor de dados:  BJM
País:  Brazil
Título:  The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
Autores:  Yao,Xie-feng
Zhang,Jiu-ming
Tian,Li
Guo,Jian-hua
Data:  2017-03-01
Ano:  2017
Palavras-chave:  Heavy metal contamination
PCR-DGGE fingerprinting
Multivariate analysis
Bacterial community structure
Resumo:  Abstract In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100071
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2016.09.007
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.48 n.1 2017
Direitos:  info:eu-repo/semantics/openAccess
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