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Provedor de dados:  BJM
País:  Brazil
Título:  Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
Autores:  Amna,Touseef
Amina,Musarat
Sharma,P.R.
Puri,S.C.
Al-Youssef,Hanan M.
Al-Taweel,Areej M.
Qazi,G. N.
Data:  2012-12-01
Ano:  2012
Palavras-chave:  Camptothecin
Entrophospora infrequens
Cancer
Nothapodytes foetida
Precursors
Resumo:  We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but fungal endopytes may be an alternative source of production. In present study we have observed the effect of different nutrient combinations and precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid and leucine) on the accumulation of camptothecin by endophytic fungus Entrophospora infrequens. The precursors were fed either alone or in combinations (tryptophan and geraniol, tryptophan and citral, tryptophan and mevalonic acid, tryptophan and leucine). The highest camptothecin content was observed in the range of 503 ± 25µg/100g dry cell mass in Sabouraud medium. Camptothecin content in the medium was increased by 2.5 folds by the presence of tryptophan and leucine whereas the production with trytophan was also significantly different from other treatments. Furthermore, the effect of fungal camptothecin on the morphology of human cancer cell lines was also studied. The treated cells showed reduction in size, condensation of nucleus and the protoplasmic extensions were reduced. All these characteristics are found in apoptotic cells.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400032
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822012000400032
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.43 n.4 2012
Direitos:  info:eu-repo/semantics/openAccess
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