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Provedor de dados: |
BJM
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País: |
Brazil
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Título: |
Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)
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Autores: |
Freschi,Carla Roberta
Carvalho,Luiz Fernando de Oliveira e Silva
Oliveira,Celso José Bruno de
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Data: |
2005-12-01
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Ano: |
2005
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Palavras-chave: |
Diagnosis
DNA
Feces
PCR
Salmonella Typhimurium
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Resumo: |
The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P<0.05) when combined to Rappaport-Vassiliadis. Considering cost and efficiency parameters, we encourage the use of Müller-Kauffmann tetrathionate broth in combination with boiling-centrifugation DNA-extraction procedure.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400011
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Editor: |
Sociedade Brasileira de Microbiologia
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Relação: |
10.1590/S1517-83822005000400011
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Formato: |
text/html
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Fonte: |
Brazilian Journal of Microbiology v.36 n.4 2005
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Direitos: |
info:eu-repo/semantics/openAccess
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