| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites
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| Autores: |
Mercimek,Hatice Aysun
Dincer,Sadik
Guzeldag,Gulcihan
Ozsavli,Aysenur
Matyar,Fatih
Arkut,Afet
Kayis,Fikret
Ozdenefe,Melis Sumengen
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| Data: |
2015-03-01
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| Ano: |
2015
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| Palavras-chave: |
Pseudomonas sp
Biotransformation
Nitroaromatic explosives
TNT
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| Resumo: |
Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitroexperiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L−1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL−1 TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100103
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-838246120140026
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.46 n.1 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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