| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
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| Autores: |
Martins,Maira de S.N.
Castro,Alessandra M.M.G. de
Lima,Michele dos S.
Pinto,Vivian da S.C.
Silva,Thaís G. da
Fava,Claudia Del
Depes,Claudio Regis
Okuda,Liria H.
Pituco,Edviges M.
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| Data: |
2017-06-01
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| Ano: |
2017
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| Palavras-chave: |
Ovine herpesvirus type 2 (OvHV-2)
Histopathology
Qualitative PCR
Quantitative PCR
Phylogenetic analysis
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| Resumo: |
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2016.10.021
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.48 n.2 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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