| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Enhancement of pigment extraction from B. braunii pretreated using CO2 rapid depressurization
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| Autores: |
Uquiche,Edgar
Antilaf,Ivette
Millao,Sonia
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| Data: |
2016-06-01
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| Ano: |
2016
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| Palavras-chave: |
Botryococcus braunii
Cell disruption
Chlorophylls
Microalgae
Supercritical extraction
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| Resumo: |
Abstract Extraction of compounds from microalgae requires cell disruption as a pretreatment to increase extraction yield. Botryococcus braunii is a microalga with a significant content of carotenoids and other antioxidant compounds, such as chlorophylls. Cell disruption of B. braunii using CO2 rapid depressurization was studied as a pretreatment for the extraction of carotenoid and chlorophyll pigments. We studied the effect of temperature (21–49 °C) and pressure (6–13 MPa) during static compression on pigment recovery with supercritical CO2 at 40 °C, 30 MPa and solvent flow of 4.7 L NPT/min. Within the experimental region, the extraction yield of carotenoids and chlorophylls increased by 2.4- and 2.2-fold respectively. Static compression conditions of high pressure and low temperature increased the extraction of carotenoids and especially chlorophylls. We selected 21 °C and 13 MPa as the cell disruption condition, which produced 1.91 g/kg d.s. of carotenoids and 14.03 mg/kg d.s. of chlorophylls. Pretreated microalga gave a 10-fold higher chlorophyll extraction yield compared to the untreated sample. While for carotenoids and tocopherols were 1.25 and 1.14-fold higher, respectively. Additionally, antioxidant activity of pretreated microalga (33.22 mmol TE/kg oil) was significantly higher than the value for the untreated samples (29.11 mmol TE/kg oil) (p ≤ 0.05). Confocal microscopy images showed morphological differences between micro-colonies with and without disruption treatment, suggesting that partial cell disruption by rapid depressurization improved the extraction of microalga compounds.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200497
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2016.01.020
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.47 n.2 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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