Registro completo |
Provedor de dados: |
BJM
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País: |
Brazil
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Título: |
A novel expression vector for the secretion of abaecin in Bacillus subtilis
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Autores: |
Li,Li
Mu,Lan
Wang,Xiaojuan
Yu,Jingfeng
Hu,Ruiping
Li,Zhen
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Data: |
2017-12-01
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Ano: |
2017
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Palavras-chave: |
Abaecin
Antimicrobial peptides
Bacillus subtilis
Expression
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Resumo: |
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809
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Editor: |
Sociedade Brasileira de Microbiologia
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Relação: |
10.1016/j.bjm.2017.01.009
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Formato: |
text/html
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Fonte: |
Brazilian Journal of Microbiology v.48 n.4 2017
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Direitos: |
info:eu-repo/semantics/openAccess
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