| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells
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| Autores: |
Jerez,José Antonio
Gregori,Fábio
Brandão,Paulo Eduardo
Rodriguez,Cesar Alejandro Rosales
Ito,Fumio Honma
Buzinaro,Maria da Glória
Sakai,Takeo
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| Data: |
2005-09-01
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| Ano: |
2005
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| Palavras-chave: |
Bovine coronavirus (BCoV)
Isolation
HmLu-1 cells
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| Resumo: |
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new methodologies should be developed for this purpose and then submitted to confirmation of isolation by means of seroneutralization.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822005000300001
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.36 n.3 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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