| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Different culture media applied to the study of Cryptococcus neoformans susceptibility to amphotericin B and fluconazole
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| Autores: |
Alves,Sydney Hartz
Oliveira,Loiva T.
Goulart,Letícia S.
Linares,Carlos Eduardo B.
Griebeler,Josiane
Santurio,Janio M.
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| Data: |
2002-01-01
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| Ano: |
2002
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| Palavras-chave: |
Cryptococcus neoformans
Susceptibility
Antifungal agents
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| Resumo: |
This study compared the performance of three culture media used for the determination of minimal inhibitory concentration (MIC) of amphotericin B and fluconazole against 48 Cryptococcus neoformans strains. For amphotericin B the media employed were RPMI 1640 medium, as indicated by NCCLS as reference, Yeast Nitrogen Base (YNB) as proposed by Ghannoum et al. (YNB-1), Antibiotic medium 3 (AM3) indicated by others, and YNB as proposed by us (YNB-2). In YNB-1, amphotericin B showed broader MIC ranges, varying from 0.125 µg/ml to 1.0 µg/ml; in RPMI 1640 medium and YNB-2 the MICs were similar and in AM3 the MIC ranges were narrower. The same pattern was observed for MIC 90%. For fluconazole the media employed were RPMI 1640 and YNB-1. The MIC ranges were broader and elevated when YNB-1 was employed. MIC 50% were 2.0 µg/ml in RPMI 1640 medium and 4.0 µg/ml in YNB-1. Our results indicate that RPMI 1640 supplemented with 2% glucose allowed a good growth at 48h; YNB as recommended by Ghannoum et al. provided a broader MIC range, AM3 provided a narrow MIC range for amphotericin B, and YNB-2 was very similar to RPMI 1640 medium.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100005
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822002000100005
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.33 n.1 2002
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| Direitos: |
info:eu-repo/semantics/openAccess
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