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Provedor de dados:  BJM
País:  Brazil
Título:  Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66
Autores:  Naveed,Muhammad
Ahmed,Iftikhar
Khalid,Nauman
Mumtaz,Abdul Samad
Data:  2014-06-01
Ano:  2014
Palavras-chave:  Glucose dehydrogenase
Pyrroloquinoline quinone
Leclercia sp
PGPR
Bioinformatics
Resumo:  Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200031
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822014000200031
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.45 n.2 2014
Direitos:  info:eu-repo/semantics/openAccess
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