| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
A quantitative analysis of Propionibacterium acnes in lesional and non-lesional skin of patients with progressive macular hypomelanosis by real-time polymerase chain reaction
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| Autores: |
Cavalcanti,Silvana Maria de Morais
França,Emmanuel Rodrigues de
Magalhães,Marcelo
Lins,Ana Kelly
Brandão,Laura Costa
Magalhães,Vera
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| Data: |
2011-06-01
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| Ano: |
2011
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| Palavras-chave: |
Hypopigmentation/progressive macular hypomelanosis
Propionibacterium acnes
Polymerase chain reaction
Biopsy/bacteriology
ROC curve
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| Resumo: |
Little is known about the etiology of progressive macular hypomelanosis, although it has been suggested that Propionibacterium acnes plays an important role. While microbiological culture is commonly employed to identify Propionibacterium acnes, new identification methods have been under investigation, amongst them polymerase chain reaction. To determine the cut-off point for the number of genome copies of Propionibacterium acnes in the lesional skin of patients with progressive macular hypomelanosis as a positive marker, employing quantitative real-time polymerase chain reaction and anaerobic culture, considered gold standard. An observational study with a comparison group, included 35 patients with dermatosis, attended at the Oswaldo Cruz University Hospital, Pernambuco, Brazil, between March and May 2008. Lesional skin was compared to non-lesional skin through positive testing with real-time polymerase chain reaction and culture. The Statistical Package for Social Sciences, version 12.0, was employed for the association analysis with the McNemar test, and the cut-off point with the ROC curve for maximum values. Propionibacterium acnes was most frequently encountered in lesional areas (p<0,025). The cut-off point of Propionibacterium acnes in lesional skin was 1,333 genome copies, with a sensitivity of 87,9% and a specificity of 100,0%. Since Propionibacterium acnes is a saprophyte, identifying the cut-off point may assist in determining its positivity in lesional skin in patients suffering with this dermatosis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200002
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822011000200002
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.42 n.2 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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