| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Cytotoxicity and anti-Sporothrix brasiliensis activity of the Origanum majorana Linn. oil
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| Autores: |
Waller,Stefanie Bressan
Madrid,Isabel Martins
Ferraz,Vanny
Picoli,Tony
Cleff,Marlete Brum
de Faria,Renata Osório
Meireles,Mário Carlos Araújo
de Mello,João Roberto Braga
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| Data: |
2016-12-01
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| Ano: |
2016
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| Palavras-chave: |
Sporotrichosis
Sporothrix schenckii complex
Antifungal resistance
Marjoram
Lamiaceae
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| Resumo: |
Abstract The study aimed to evaluate the anti-Sporothrix sp. activity of the essential oil of Origanum majorana Linn. (marjoram), its chemical analysis, and its cytotoxic activity. A total of 18 fungal isolates of Sporothrix brasiliensis (n: 17) from humans, dogs and cats, and a standard strain of Sporothrix schenckii (n: 1) were tested using the broth microdilution technique (Clinical and Laboratory Standard Institute - CLSI M27-A3) and the results were expressed in minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). The MIC50 and MIC90 of itraconazole against S. brasiliensis were 2 µg/mL and 8 µg/mL, respectively, and the MFC50 and MFC90 were 2 µg/mL and >16 µg/mL, respectively, with three S. brasiliensis isolates resistant to antifungal. S. schenckii was sensitive at MIC of 1 µg/mL and MFC of 8 µg/mL. For the oil of O. majorana L., all isolates were susceptible to MIC of ≤2.25-9 mg/mL and MFC of ≤2.25-18 mg/mL. The MIC50 and MIC90 were ≤2.25 mg/mL and 4.5 mg/mL, respectively, and the MFC50/90 values were twice more than the MIC. Twenty-two compounds were identified by gas chromatography with a flame ionization detector (CG-FID) and 1,8-cineole and 4-terpineol were the majority. Through the colorimetric (MTT) assay, the toxicity was observed in 70-80% of VERO cells between 0.078 and 5 mg/mL. For the first time, the study demonstrated the satisfactory in vitro anti-Sporothrix sp. activity of marjoram oil and further studies are needed to ensure its safe and effective use.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400896
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2016.07.017
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.47 n.4 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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