| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA
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| Autores: |
Ono,Ekaterina Alexandrovna Durymanova
Taniwaki,Sueli Akemi
Brandão,Paulo
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| Data: |
2017-07-01
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| Ano: |
2017
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| Palavras-chave: |
Rabies
Treatment
RNA interference
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| Resumo: |
Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce partial protection, but with no significant difference when compared to non-treated mice. These results indicate that, despite the need for improvement for in vivo applications, P mRNA might be a target for an RNAi-based treatment for rabies.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300566
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2016.11.007
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.48 n.3 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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