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Provedor de dados:  BJM
País:  Brazil
Título:  Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil
Autores:  Vidotto,Marilda C.
Lima,Natália C.S. de
Fritzen,Juliana T.T.
Freitas,Júlio C. de
Venâncio,Emerson J.
Ono,Mario A.
Data:  2009-03-01
Ano:  2009
Palavras-chave:  Porcine Escherichia coli
Virulence genes
Postweaning diarrhea
Toxins
Resumo:  Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100035
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822009000100035
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.40 n.1 2009
Direitos:  info:eu-repo/semantics/openAccess
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