| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
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| Autores: |
Rezende,Maria Inês
Barbosa,Aneli de Melo
Vasconcelos,Ana Flora Dalberto
Endo,Asae Sakurada
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| Data: |
2002-01-01
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| Ano: |
2002
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| Palavras-chave: |
Trichoderma harzianum
Xylanase
Solid state fermentation
Sugarcane bagasse
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| Resumo: |
Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and sugarcane bagasse concentration, on xylanase production were also investigated. The growth profile of Trichoderma harzianum was followed over 20 days on 14% (w/v) bagasse, and highest xylanase activity (288U/ml) appeared on the seventh day. The enzymatic extract after precipitation with ammonium sulphate was submitted to electrophoresis on polyacrylamide gels and showed 4 protein-staining bands, one of which exhibited xylanase activity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822002000100014
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.33 n.1 2002
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| Direitos: |
info:eu-repo/semantics/openAccess
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