| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil
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| Autores: |
Leite,Clariana Akemi Kariya
Oizumi,Karina Yoshimi
Caleffi-Ferracioli,Katiany Rizzieri
Scodro,Regiane Bertin de Lima
Pádua,Rubia Andreia Falleiros de
Cardoso,Rosilene Fressatti
Pires,Claudia Terencio Agostinho
Siqueira,Vera Lucia Dias
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| Data: |
2017-01-01
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| Ano: |
2017
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| Palavras-chave: |
Enterobacteriaceae
Pseudomonas aeruginosa
Acinetobacter spp
Antimicrobial resistance
Beta-lactamases
Bacterial typing
Intensive Care Unit/study/Brazil
Drug-resistant bacteria.
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| Resumo: |
ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic extended spectrum β-lactamase (ESBL) test was positive in 53.8% of the K. pneumoniae isolates, 100.0% of the E. coli isolates, and 100.0% of the E. aerogenes isolates. Phenotypic and genotypic testing of K. pneumoniae carbapenemase (KPC) was positive in 50.0% of the K. pneumoniae isolates. Phenotypic and genotypic testing showed that none of the P. aeruginosa or Acinetobacter spp. isolates were positive for metallo- β-lactamase (MBL). The bla OXA gene was detected only in Acinetobacter spp. The lowest genetic diversity, determined by ERIC-PCR, was observed among the KPC-producing K. pneumoniae isolates and OXA-producing Acinetobacter spp. isolates, indicating the inadequate dissemination control of multidrug-resistant bacteria in this hospital environment.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/s2175-97902017000216111
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.53 n.2 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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