| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
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| Autores: |
Silva,Pedro Henrique Reis da
Enéas,Paula Cristina Rezende
Fernandes,Christian
Pianetti,Gerson Antônio
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| Data: |
2017-01-01
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| Ano: |
2017
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| Palavras-chave: |
Prednisone
Capsules
High performance liquid chromatography/validation
Linearity assessment.
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| Resumo: |
ABSTRACT Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD<2.0%), robustness, and accuracy (average recovery of 100.05%). The chromatographic procedure was applied for assay and uniformity content determination of three different batches of prednisone capsules, showing to be suitable for their quality control.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200624
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/s2175-97902017000216064
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.53 n.2 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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