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Provedor de dados:  BJPS
País:  Brazil
Título:  Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant
Autores:  Lee,Sarah Hwa In
Barancelli,Giovana Verginia
Corassin,Carlos Humberto
Rosim,Roice Eliana
Coppa,Carolina Fernanda Sengling Cebin
Oliveira,Carlos Augusto Fernandes de
Data:  2017-01-01
Ano:  2017
Palavras-chave:  Peracetic acid (PAA)/ biofilm/effects
L. monocytogenes
Dairy plants/Brazil
Resumo:  ABSTRACT This study aimed to investigate the effect of peracetic acid (PAA, 0.5%) on adherent cells of three strains of Listeria monocytogenes strains belonging to serotypes 4b and 1/2b that had been previously isolated from the environment of a Brazilian cheese plant. The assays were conducted using polystyrene microplates and stainless steel coupons and the adhered cells were treated with PAA for 60, 120 and 180 s. On stainless steel, biofilms were partially inactivated by PAA after 60 s and almost 100% of the cells were damaged within 180 s using epifluorescence microscopy with LIVE/DEAD® staining. On polystyrene microplates, PAA decreased (P<0.05) biofilm biomass produced by the three L. monocytogenes isolates at 60 s, when compared with controls (no PAA treatment). However, PAA did not completely eliminate L. monocytogenes cells on polystyrene microplates (decreasing 1.8-2.5 log cycles after treatment with PAA for 180 s). The correct concentration and contact time of PAA is critical for eliminating biofilms formed by L. monocytogenes on stainless steel surfaces, although further studies are needed for defining efficient PAA treatments to remove adherent cells of this pathogen on plastic polymers.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300610
Editor:  Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação:  10.1590/s2175-97902017000300071
Formato:  text/html
Fonte:  Brazilian Journal of Pharmaceutical Sciences v.53 n.3 2017
Direitos:  info:eu-repo/semantics/openAccess
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