Registro completo |
Provedor de dados: |
BJPS
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País: |
Brazil
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Título: |
Simultaneous determination of abamectin homologs H 2 B 1a and H 2 B 1b in gel formulation by high performance liquid chromatography
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Autores: |
Alexandre,Grazielle Prado
Aurora-Prado,María Segunda
Español Mariño,Laura Victoria
Singh,Anil Kumar
Leite,Helen Dutra
Kedor-Hackmann,Erika Rosa Maria
Santoro,Maria Inês Rocha Miritello
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Data: |
2016-09-01
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Ano: |
2016
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Palavras-chave: |
Abamectin homologs/validation method/gel formulation
Abamectin homologs
High Perfomance Liquid Chromatography (HPLC).
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Resumo: |
ABSTRACT Abamectin is a drug with antiparasitic properties used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for quantification of the two abamectin homologs (H2B1a and H2B1b) in gel formulation. This HPLC method was validated using a LichroCart(r) 100 RP-18 (125 x 4 mm, 5 µm) column. The mobile phase contained of acetonitrile and water (95:5 v/v) with 1% acetic acid. The flow rate was 1.0 mL min-1 and UV detection was performed at 245 nm. Mobile phase solutions were prepared containing a nominal concentration 185.2 µg mL-1 H2B1a and 9.6 µg mL-1 H2B1b. The method displayed good linearity in the concentration range of 148.1 - 222.3 µg mL-1 and 7.7 - 11.5 µg mL-1, for H2B1a and H2B1b, respectively, with a correlation coefficient of (r)> 0.99 for both compounds, calculated by the least mean squares method. Detection limits (DLs) were 2.8 µg mL-1 and 1.2 µg mL-1 and quantitation limits (QLs) were 8.6 µg mL-1 and 3.8 µg mL-1, for H2B1a and H2B1b, respectively. The method is simple, economical and efficient for the quantitative determination of abamectin H2B1a and H2B1b homologs in pharmaceutical preparations.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300509
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Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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Relação: |
10.1590/s1984-82502016000300016
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Formato: |
text/html
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Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.52 n.3 2016
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Direitos: |
info:eu-repo/semantics/openAccess
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